Antioxidant Activity and Bioactive Compounds of Lamium album Flower Extracts Obtained by Supercritical Fluid Extraction
نویسندگان
چکیده
In this research, supercritical CO2 extraction is applied to extract bioactive compounds from Lamium album (white dead nettle, Lamiaceae). Extraction was performed at various temperatures (40, 50, and 60 °C) using methanol as co-solvent a constant flow rate of CO2, methanol, pressure. The collected extracts were characterized in terms antioxidant capacity by DPPH, ABTS FRAP vitro activity assays, whereas the Folin–Ciocalteu procedure employed estimate total phenols content (TPC). On other hand, phenolic quantitated liquid chromatography coupled with photodiode array detector (UPLC-PDA) confirmed mass (TQD). have shown high TPC ranged between 234.17 650.17 mg GAE/g extract. DPPH scavenging estimated obtained EC50 values 0.12 0.37 mg/mL solution. radical 43.20 44.53 µg TE/g. value found within range 19.48 44.74 µmol TE/g Differences conditions observed. 50 °C/250 bar efficient for TPC, ABTS, assays; moreover, statistically, TPCs assay showed significant differences ? = 0.05. identification flowers, UPLC/PDA, revealed that chrysin, pinostrobin, myricetin, trans-3-hydroxycinnamic acid molecules present, which may be responsible polyphenols activity. results indicated SC-CO2 could considered an alternative method extracting album. High presence indicate potential plant Lamiaceae family possibility its application industries, including agriculture, food technology, or pharmacy.
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ژورنال
عنوان ژورنال: Applied sciences
سال: 2021
ISSN: ['2076-3417']
DOI: https://doi.org/10.3390/app11167419